It's from Saga #25, which is my favorite comic book in the world right now.
It's the look of a father, estranged from his toddler daughter, who is not sure where his daughter is, when he might see her again, or whether she and her mother are in immediate danger. But I didn't need to tell you any of that, becasue you can see all of that, and more, in Marko's eyes.
It's all right there. This panel is what makes Saga transcendent.